• Product NameGRAH Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total GRAH protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human GRAH.
• Target NameGRAH
• Alternative Names    Granzyme H [Precursor]; EC 3.4.21.-; Cytotoxic T-lymphocyte proteinase; Cathepsin G-like 2; CTSGL2
• Accession No. Swiss-Prot: P20718
  NCBI Gene ID: 2999
• UniprotP20718
• Gene ID2999;
• SDS-PAGE MW 23kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.

Meier M., Biochemistry 29:4042-4049(1990).
Haddad P., Int. Immunol. 3:57-66(1991).
Klein J.L., Tissue Antigens 35:220-228(1990).

• Western blot analysis of extracts from K562 cells, using GRAH antibody #34271.
• Immunofluorescence analysis of A549 cells, using GRAH antibody #34271.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting