• Product NameMGMT Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous levels of total MGMT protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MGMT.
• Target NameMGMT
• Alternative Names    Methylated-DNA--protein-cysteine methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; MGMT;
• Accession No. Swiss-Prot: P16455
  NCBI Gene ID: 4255
• UniprotP16455
• Gene ID4255;
• SDS-PAGE MW 22kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Tano K., Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990).
Rydberg B., J. Biol. Chem. 265:9563-9569(1990).
Koike G., J. Biol. Chem. 265:14754-14762(1990)

• Western blot analysis of extracts from Jurkat cells, using MGMT antibody #34286.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting