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Elk1(Phospho-Ser389) Antibody

11037
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  Catalog No. Package Size Price
11037-1 50ul $189.00
11037-2 100ul $279.00

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Description
  • Product Name Elk1(Phospho-Ser389) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of Elk1 only when phosphorylated at serine 389.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 389 (P-R-S(p)-P-A) derived from Human Elk-1.
  • Target Name Elk1
  • Modification Phospho
  • Alternative Names ELK1; ETS-domain protein Elk-1;
  • Accession No. Swiss-Prot: P19419
    NCBI Protein: NP_001107595.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 45kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • Elk1(Phospho-Ser389) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1(Phospho-Ser389) Antibody #11037(left) or the same antibody preincubated with blocking peptide(right).

  • Elk1(Phospho-Ser389) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from A549 cells untreated or treated with TNFa; HT29 and Hela cells untreated or treated withUV using Elk1(Phospho-Ser389) Antibody #11037

Background

Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene

Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104.
Marais R, et al. (1993) Cell 73:381-393.
Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824.
Hill C S, et al. (1995) Cell. 80:199-211.
Cavigelli M, et al. (1995) EMBO J. 14:5957-5964.

    Please let us know if you have published research using #11037 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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