• Product NameBCAS2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total BCAS2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human BCAS2.
• Target NameBCAS2
• Alternative Names    Pre-mRNA-splicing factor SPF27; Spliceosome-associated protein SPF 27; Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; BCAS2
• Accession No. Swiss-Prot: O75934
  NCBI Gene ID: 10286
• UniprotO75934
• Gene ID10286;
• SDS-PAGE MW 27kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.

Neubauer G., Nat. Genet. 20:46-50(1998).
Nagasaki K., Cancer Lett. 140:219-226(1999).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

• Western blot analysis of extracts from HeLa cells, HepG2 cells, Huvec cells, A549 cells, K562 cells and Jurkat cells, using BCAS2 antibody #34508.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting