• Product NameC1QB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total C1QB protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human C1QB.
• Target NameC1QB
• Alternative Names    complement C1q subcomponent B; complement component 1; q subcomponent; B;
• Accession No. Swiss-Prot: P02746
  NCBI Gene ID: 713
• UniprotP02746
• Gene ID713;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca2+-dependent C1r2C1s2 proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Reid K.B.M.; Biochem. J. 231:729-735(1985).
Gregory S.G., Nature 441:315-321(2006).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from Jurkat cells, using C1QB antibody #34607.
• Immunofluorescence analysis of A549 cells, using C1QB antibody #34607.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting