• Product NameNCAPH Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NCAPH protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human NCAPH.
• Target NameNCAPH
• Alternative Names    Condensin complex subunit 2; Non-SMC condensin I complex subunit H; Barren homolog protein 1; Chromosome-associated protein H; hCAP-H
• Accession No. Swiss-Prot: Q15003
  NCBI Gene ID: 23397
• UniprotQ15003
• Gene ID23397;
• SDS-PAGE MW 83kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

Nomura N., DNA Res. 1:223-229(1994).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Cabello O.A., Mol. Biol. Cell 12:3527-3537(2001).

• Western blot analysis of extracts from COLO cells, using NCAPH antibody #34615.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting