• Product NameDOCK1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DOCK1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DOCK1.
• Target NameDOCK1
• Alternative Names    180 kDa protein downstream of CRK; dedicator of cytokinesis protein 1; DOC1; DOCK180;
• Accession No. Swiss-Prot: Q14185
  NCBI Gene ID: 1793
• UniprotQ14185
• Gene ID1793;
• SDS-PAGE MW 215kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.

Hasegawa H., Mol. Cell. Biol. 16:1770-1776(1996).
Deloukas P., Nature 429:375-381(2004).
Matsuda M., J. Biol. Chem. 271:14468-14472(1996).

• Western blot analysis of extracts from HeLa cells, using DOCK1 antibody #34642.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting