• Product NameRNF138 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RNF138 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human RNF138.
• Target NameRNF138
• Alternative Names    E3 ubiquitin-protein ligase RNF138; EC 6.3.2.-; RING finger protein 138; Nemo-like kinase-associated RING finger protein; NLK-associated RING finger protein
• Accession No. Swiss-Prot: Q8WVD3
  NCBI Gene ID: 51444
• UniprotQ8WVD3
• Gene ID51444;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.

Wang L., Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

• Western blot analysis of extracts from MCF-7 cells, using RNF138 antibody #34663.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting