• Product NameUBR1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total UBR1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human UBR1.
• Target NameUBR1
• Alternative Names    EC 6.3.2.-; Ubiquitin ligase E3 alpha-I;
• Accession No. Swiss-Prot: Q8IWV7
  NCBI Gene ID: 197131
• UniprotQ8IWV7
• Gene ID197131;
• SDS-PAGE MW 36kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth.

Kwak K.S., Cancer Res. 64:8193-8198(2004).
Dgany O., Am. J. Hum. Genet. 71:1467-1474(2002).
Ota T., Nat. Genet. 36:40-45(2004).

• Western blot analysis of extracts from Jurkat cells and HepG2 cells, using UBR1 antibody #34664.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting