• Product NameMAN1B1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MAN1B1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MAN1B1.
• Target NameMAN1B1
• Alternative Names    Endoplasmic reticulum mannosyl-oligosaccharide 1; 2-alpha-mannosidase; EC=3.2.1.113; ER alpha-1; 2-mannosidase
• Accession No. Swiss-Prot: Q9UKM7
  NCBI Gene ID: 11253
• UniprotQ9UKM7
• Gene ID11253;
• SDS-PAGE MW 80kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2.

Tremblay L.O., Glycobiology 9:1073-1078(1999) [PubMed: 10521544].
Gonzalez D.S., J. Biol. Chem. 274:21375-21386(1999) [PubMed: 10409699].
Clark H.F., Genome Res. 13:2265-2270(2003) [PubMed: 12975309].

• Western blot analysis of extracts from 3T3 cells, using MAN1B1 antibody #34675.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting