• Product NameGCF Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total GCF protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human GCF.
• Target NameGCF
• Alternative Names    C2orf3; GC-rich sequence DNA-binding factor; GCF; TCF-9; Transcription factor 9
• Accession No. Swiss-Prot: P16383
  NCBI Gene ID: 6936
• UniprotP16383
• Gene ID6936;
• SDS-PAGE MW 80kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters. Involved in pre-mRNA splicing through regulating spliceosome C complex formation. May play a role during late-stage splicing events and turnover of excised inrons.

Kageyama R., Cell 59:815-825(1989).
Hillier L.W., Nature 434:724-731(2005).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from RAW264.7 cells, using GCF antibody #34702.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting