• Product NameLFA3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total LFA3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human LFA3.
• Target NameLFA3
• Alternative Names    Lymphocyte function-associated antigen 3; Ag3; Surface glycoprotein LFA-3; CD58 antigen;
• Accession No. Swiss-Prot: P19256
  NCBI Gene ID: 965
• UniprotP19256
• Gene ID965;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.

Wallner B.P., J. Exp. Med. 166:923-932(1987).
Wallich R., J. Immunol. 160:2862-2871(1998).
Ota T., Nat. Genet. 36:40-45(2004).

• Western blot analysis of lysates from COS7 cells, using GTPase Activating Protein (Phospho-Ser387) Antibody. The lane on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting