• Product NameMLF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MLF1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MLF1.
• Target NameMLF1
• Alternative Names    Myeloid leukemia factor 1; Myelodysplasia-myeloid leukemia factor 1;
• Accession No. Swiss-Prot: P58340
  NCBI Gene ID: 4291
• UniprotP58340
• Gene ID4291;
• SDS-PAGE MW 30kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.

Yoneda-Kato N., Oncogene 12:265-275(1996) [PubMed: 8570204].
Hanissian S.H., Oncogene 23:3700-3707(2004) [PubMed: 15116101].
Yoneda-Kato N., EMBO J. 24:1739-1749(2005) [PubMed: 15861129].

• Western blot analysis of extracts from HUVEC cells, using MLF1 antibody #34814.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting