• Product NameMT-ND5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MT-ND5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MT-ND5.
• Target NameMT-ND5
• Alternative Names    NADH-ubiquinone oxidoreductase chain 5; EC=1.6.5.3; NADH dehydrogenase subunit 5; MTND5; NADH5
• Accession No. Swiss-Prot: P03915
  NCBI Gene ID: 4540
• UniprotP03915
• Gene ID4540;
• SDS-PAGE MW 70kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.

Anderson S., Nature 290:457-465(1981).
Horai S., Proc. Natl. Acad. Sci. U.S.A. 92:532-536(1995).
Maca-Meyer N., BMC Genet. 2:13-13(2001).

• Western blot analysis of extracts from HT-29 cells, using MT-ND5 antibody #34840.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting