• Product NameARHGEF5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ARHGEF5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ARHGEF5.
• Target NameARHGEF5
• Alternative Names    ARH5; guanine nucleotide regulatory protein TIM; Oncogene TIM; p60 TIM; Rho guanine nucleotide exchange factor 5
• Accession No. Swiss-Prot: Q12774
  NCBI Gene ID: 7984
• UniprotQ12774
• Gene ID7984;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.

Chan A.M.-L., Oncogene 9:1057-1063(1994).
Jikuya H., Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W., Nature 424:157-164(2003)

• Western blot analysis of extracts from RAW264.7 cells, using ARHGEF5 antibody #34996.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting