• Product NameMRRF Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MRRF protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MRRF.
• Target NameMRRF
• Alternative Names    Ribosome-recycling factor; mitochondrial; RRF; Ribosome-releasing factor; mitochondrial
• Accession No. Swiss-Prot: Q96E11
  NCBI Gene ID: 92399
• UniprotQ96E11
• Gene ID92399;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another By similarity.

Ota T., Nat. Genet. 36:40-45(2004).
Humphray S.J., Nature 429:369-374(2004).
Zhang Y., Biochim. Biophys. Acta 1443:245-250(1998).

• Western blot analysis of extracts from HeLa cells, using MRRF antibody #35003.
• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using MRRF antibody #35003.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting