• Product NameRFWD3Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RFWD3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human RFWD3.
• Target NameRFWD3
• Alternative Names    MLKL; ring finger and WD repeat domain 3; RING finger protein 201; RNF201;
• Accession No. Swiss-Prot: Q6PCD5
  NCBI Gene ID: 55159
• UniprotQ6PCD5
• Gene ID55159;
• SDS-PAGE MW 85kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and repair.

The MGC Project Team, Genome Res. 14:2121-2127(2004).
Ota T., Nat. Genet. 36:40-45(2004).
Matsuoka S., Science 316:1160-1166(2007).

• Western blot analysis of extracts from MCF-7 cells, using RFWD3 antibody #35004.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting