• Product NameEWSR1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total EWSR1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human EWSR1.
• Target NameEWSR1
• Alternative Names    EWS oncogene; Ewing sarcoma breakpoint region 1 protein;
• Accession No. Swiss-Prot: Q01844
  NCBI Gene ID: 2130
• UniprotQ01844
• Gene ID2130;
• SDS-PAGE MW 68kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Might normally function as a transcriptionnal repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Delattre O., Nature 359:162-165(1992).
Plougastel B., Genomics 18:609-615(1993).
Zucman-Rossi J., Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.

• Western blot analysis of extracts from HeLa cells, Jurkat cells and K562 cells, using EWSR1 antibody #35008.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting