• Product NameSHB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SHB protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human SHB.
• Target NameSHB
• Alternative Names    SH2 domain-containing adapter protein B; SHB;
• Accession No. Swiss-Prot: Q15464
  NCBI Gene ID: 6461
• UniprotQ15464
• Gene ID6461;
• SDS-PAGE MW 55kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.

Welsh M., Oncogene 9:19-27(1994).
Humphray S.J., Nature 429:369-374(2004).
Mural R.J., Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

• Western blot analysis of extracts from HuvEc cells, using SHB antibody #35039.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting