• Product NameANAPC5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ANAPC5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ANAPC5.
• Target NameANAPC5
• Alternative Names    ANAPC5; Anaphase-promoting complex subunit 5; ANC5; Similar to anaphase-promoting complex subunit 5;
• Accession No. Swiss-Prot: Q9UJX4
  NCBI Gene ID: 51433
• UniprotQ9UJX4
• Gene ID51433;
• SDS-PAGE MW 85kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Yu H., Science 279:1219-1222(1998).
Bechtel S., BMC Genomics 8:399-399(2007).
Kraft C., EMBO J. 22:6598-6609(2003).

• Western blot analysis of extracts from HeLa cells, Jurkat cells and COLO205 cells, using ANAPC5 antibody #35242.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting