• Product NameBCL2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total BCL2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 1 and 125 of BCL2.
• Target NameBCL2
• Alternative Names    Bcl-2 antibody; BCL2 antibody; "protein phosphatase 1; regulatory subunit 50 antibody"; apoptosis regulator Bcl-2 antibody; B-cell CLL/lymphoma 2 antibody
• Accession No. Swiss-Prot#:P10415;
  NCBI Gene#:596
• UniprotP10415
• Gene ID596;
• SDS-PAGE MW 26kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:10000
  

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: Raji
  12% SDS PAGE
  #35342 diluted at 1:1000
  
• Sample (30 ug of whole cell lysate)
  A: Non-transfected 293T lysates
  B: BCL2 transfected 293T lysate
  12% SDS PAGE
  #35342 diluted at 1:5000
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting