• Product NameCD14 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CD14 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 1 and 177 of CD14.
• Target NameCD14
• Alternative Names    CD14 antibody; myeloid cell-specific leucine-rich glycoprotein antibody; monocyte differentiation antigen CD14 antibody; CD14 molecule antibody
• Accession No. Swiss-Prot#:P08571;
  NCBI Gene#:929
• UniprotP08571
• Gene ID929;
• SDS-PAGE MW 40kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  

CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq]

• Sample(30 μg of whole cell lysate)
  A:293T
  B:A431
  C:H1299
  10% SDS PAGE
  #35368 diluted at 1:1500

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting