• Product NameACAT1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsIF WB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ACAT1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 1 and 151 of ACAT1.
• Target NameACAT1
• Alternative Names    ACAT antibody; MAT antibody; T2 antibody; THIL antibody; ACAT1 antibody; mitochondrial acetoacetyl-CoA thiolase antibody; acetoacetyl-CoA thiolase antibody; acetyl-Coenzyme A acetyltransferase 1 antibody; "acetyl-CoA acetyltransferase; mitochondrial antib
• Accession No. Swiss-Prot#:P24752;
  NCBI Gene#:38
• UniprotP24752
• Gene ID38;
• SDS-PAGE MW 45kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:1000

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq]

• Sample (50 ug of whole cell lysate)
  A: Mouse brain
  10% SDS PAGE
  #35395 diluted at 1:1000
  
• Sample(30 μg of whole cell lysate)
  A:293T
  B:A431
  C:MOLT4
  10% SDS PAGE
  #35395 diluted at 1:500
• ACAT1 antibody detects ACAT1 protein at mitochondria by immunofluorescent analysis.
  Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37˚C for 30 min.
  Green: ACAT1 protein stained by ACAT1 antibody (#35395) diluted at 1:500.
  Blue: Hoechst 33342 staining.
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting