• Product NameBLMH Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous levels of total BLMH protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 60 and 268 of BLMH.
• Target NameBLMH
• Alternative Names    BH antibody; BMH antibody; BLMH antibody; bleomycin hydrolase antibody; BLM hydrolase antibody
• Accession No. Swiss-Prot#:Q13867;
  NCBI Gene#:642
• UniprotQ13867
• Gene ID642;
• SDS-PAGE MW 53kd
• Concentration 0.87mg/ml
• Formulation Rabbit IgG in 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• WB 1:500 - 1:2000

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq]

• Western blot analysis of extracts of BT-474 cells, using CD177 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting