• Product NamePNK Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PNK protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 1 and 273 of PNK.
• Target NamePNK
• Alternative Names    EIEE10 antibody; MCSZ antibody; PNK antibody; PNKP antibody; DNA 5'-kinase/3'-phosphatase antibody; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP) antibody; bifunctional polynucleotide phosphatase/kinase antibody; polynucleotide kinase 3'-phosph
• Accession No. Swiss-Prot#:Q96T60;
  NCBI Gene#:11284
• UniprotQ96T60
• Gene ID11284;
• SDS-PAGE MW 57kd
• Concentration 0.73mg/ml
• Formulation Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  

This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.

• Sample (30 ug of whole cell lysate)
  A: Hela
  B: Hep G2
  7.5% SDS PAGE
  PNK antibody
  #35440 diluted at 1:1000
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting