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ACTB Antibody

35532
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  Catalog No. Package Size Price
35532 100ul $279.00

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Description
  • Product Name ACTB Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous levels of total ACTB protein.
  • Immunogen Type Peptide
  • Immunogen Description Synthetic peptide corresponding to residues near the N terminal of human Actin, beta
  • Target Name ACTB
  • Alternative Names BRWS1; PS1TP5BP1
  • Accession No. Swiss-Prot#: P60709
    NCBI Gene ID: 60
    Gene Accssion: NP_001092
  • SDS-PAGE MW 42kd
  • Concentration 1.6mg/ml
  • Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:1000-1:5000

Images
  • ACTB Antibody - SAB | Signalway Antibody

    Gel: 10%SDS-PAGE
    Lysates (from left to right): Hela, Jurkat and NIH/3T3 cell, human fetal liver and mouse brain tissue, A431 and 293T cell
    Amount of lysate: 40ug per lane
    Primary antibody: 1/600 dilution
    Secondary antibody dilution: 1/8000
    Exposure time: 7 seconds

Background
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs.
    Please let us know if you have published research using #35532 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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