• Product NameCSF2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total CSF2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human Colony stimulating factor 2 (granulocyte-macrophage)
• Target NameCSF2
• Alternative Names    GMCSF
• Accession No. Swiss-Prot#: P04141
  NCBI Gene ID: 1437
  Gene Accssion: BC113999
• UniprotP04141
• Gene ID1437;
• SDS-PAGE MW 16kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.

• Gel: 12%SDS-PAGE
  Lysate: 40ug RAW264.7 cell
  Primary antibody: 1/500 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 3 minutes
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #35758 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting