• Product NameCASC3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CASC3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human ancer susceptibility candidate 3
• Target NameCASC3
• Alternative Names    BTZ; MLN51
• Accession No. Swiss-Prot#: O15234
  NCBI Gene ID: 22794
  Gene Accssion: BC050526/O15234
• UniprotO15234
• Gene ID22794;
• SDS-PAGE MW 76kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

The product of this gene is a core component of the exon junction complex (EJC), a protein complex that is deposited on spliced mRNAs at exon-exon junctions and functions in nonsense-mediated mRNA decay (NMD). The encoded protein binds RNA and interacts with two other EJC core components. It is predominantly located in the Cytoplasm, but shuttles into the nucleus where it localizes to nuclear speckles.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): 293T and A549 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #36311 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting