Product NameVEGFR2(Phospho-Tyr1214) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of VEGFR2 only when phosphorylated at tyrosine 1214.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 1214 (F-H-Y(p)-D-N) derived from Human VEGFR2.

Target NameVEGFR2

ConjugateUnconjugated

Other NamesFLK1; KDR; VGFR2; VGR2; kinase insert domain receptor

Accession NoSwiss-Prot: P35968
NCBI Protein: NP_002244.1

Uniprot P35968

Gene ID 3791;

Calculated MW152kDa

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 230kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200