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VEGFR2(Phospho-Tyr951) Antibody#11086

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Product Detail

Product NameVEGFR2(Phospho-Tyr951) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of VEGFR2 only when phosphorylated at tyrosine 951.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 951 (K-D-Y(p)-V-G) derived from Human VEGFR2.

Target NameVEGFR2

ConjugateUnconjugated

Other NamesFLK1; KDR; VGFR2; VGR2; kinase insert domain receptor

Accession NoSwiss-Prot: P35968
NCBI Protein: NP_002244.1

Uniprot P35968

Gene ID 3791;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 230kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from G-CSF-treated HUVEC and serum-treated HT29 cells using VEGFR2(Phospho-Tyr951) Antibody #11086.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2(Phospho-Tyr951) Antibody #11086(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using VEGFR2(Phospho-Tyr951) Antibody #11086.
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions

Zeng H, et al. (2001) J Biol Chem. 276(35): 32714-32719.
Dougher M, et al. (1999) Oncogene. 18(8): 1619-1627.

If you have published an article using product 11086, please notify us so that we can cite your literature.

et al,CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development.In Cell Res on 2015 Mar by Wei Chen, Yan Liu et al..PMID:25656845 , (2015),
PMID: 25656845
et al,Possible involvement of VEGF signaling system in rescuing effect of endogenous acetylcholine on NMDA-induced long-lasting hippocampal cell damage in organotypic hippocampal slice cultures. In Neurochem Int on 2014 Sep by Chikako Inada, Yimin Niu,et al..PMID:24911952 , (2014),
PMID: 24911952
et al,Evidence for pro-angiogenic functions of VEGF-Ax. In Cell on 2016 Sep 22 by Hong Xin, Cuiling Zhong,et al..PMID:27662093 , (2016),
PMID: 27662093

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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