Product Detail
Product NameMyc(Ab-58) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Myc protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.56~60 (L-P-T-P-P) derived from Human Myc.
Target NameMyc
ConjugateUnconjugated
Other Namesc-myc
Accession NoSwiss-Prot: P01106
NCBI Protein: NP_002458.2
Uniprot
P01106
Gene ID
4609;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from MCF cells using Myc(Ab-58) Antibody #21034.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc(Ab-58) Antibody #21034(left) or the same antibody preincubated with blocking peptide(right).
Myc proto-oncogene encodes nuclear DNA-binding phosphoproteins that are involved in the regulation of gene expression and DNA replication during cell growth and differentiation. Myc encodes a protein of 65 kDa which is expressed in almost all normal and transformed cells. The expression correlates with the proliferation state of the cells. Transcription is repressed in quiescent or terminally differentiated cells. Expression of Myc is generally induced after mitogenic stimulation of cells or serum induction. Myc therefore is an important positive regulator of cell growth and proliferation. Myc has been demonstrated also to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. Apoptosis may serve also as a protective mechanism to prevent tumorigenicity elicited by deregulated Myc expression. Sequences of the Myc oncogene have been highly conserved throughout evolution, from Drosophila to vertebrates
Jin Z, et al. (2004) J Biol Chem. 279(38): 40209-40219.
Welcker M, et al. (2004) Proc Natl Acad Sci U S A. 101(24): 9085-9090.
Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.
Blackwood E M, et al. (1991) Science. 251:1211-1217.
Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.
If you have published an article using product 21034, please notify us so that we can cite your literature.
et al,NMDA receptor signaling mediates the expression of protein inhibitor of activated STAT1 (PIAS1) in rat hippocampus.
, (2013),
PMID:
22982248
et al,NMDA receptor signaling mediates the expression of protein inhibitor of activated STAT1 (PIAS1) in rat hippocampus. In Neuropharmacology on 2013 Feb by S Y Liu, Y L Ma, et al..PMID: 22982248
, (2013),
PMID:
22982248
et al,MiRNA-29a as a tumor suppressor mediates PRIMA-1Met-induced anti-myeloma activity by targeting c-Myc. In Oncotarget on 2016 Feb 9 by Manujendra N Saha, Jahangir Abdi, et al..PMID: 26771839
, (2016),
PMID:
26771839
Yes
