Product Detail
Product NameHIF-2 alpha Rabbit mAb
Clone No.JB24-42
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB,IHC,FC
Species ReactivityHu, Ms, Rt
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesBasic helix loop helix PAS protein MOP2 antibody
Basic-helix-loop-helix-PAS protein MOP2 antibody
bHLHe73 antibody
Class E basic helix-loop-helix protein 73 antibody
ECYT4 antibody
Endothelial PAS domain containing protein 1 antibody
Endothelial pas domain protein 1 antibody
Endothelial PAS domain-containing protein 1 antibody
EPAS 1 antibody
EPAS-1 antibody
EPAS1 antibody
EPAS1_HUMAN antibody
HIF 1 alpha like factor antibody
HIF 2 alpha antibody
HIF-1-alpha-like factor antibody
HIF-2-alpha antibody
HIF2-alpha antibody
HIF2A antibody
HLF antibody
Hypoxia inducible factor 2 alpha antibody
Hypoxia inducible factor 2 alpha subunit antibody
Hypoxia-inducible factor 2-alpha antibody
Member of PAS protein 2 antibody
Member of pas superfamily 2 antibody
MOP 2 antibody
MOP2 antibody
PAS domain-containing protein 2 antibody
PASD2 antibody
Accession NoSwiss-Prot#:Q99814
Uniprot
Q99814
Gene ID
2034;
Calculated MW96 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of HIF-2 alpha on SiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
Flow cytometric analysis of HUVEC cells with HIF-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.
If you have published an article using product 49814, please notify us so that we can cite your literature.
et al,Obstructive Sleep Apnea Affects Lacrimal Gland Function. In Invest Ophthalmol Vis Sci on 2022 Mar 2 by Shaopan Wang, Xin He,et al..PMID:35238868
, (2022),
PMID:
35238868
et al,Roxadustat ameliorates experimental colitis in mice by regulating macrophage polarization through increasing HIF levelInBiochim Biophys Acta Gen SubjOn2023 Dec 27byGuiping Kong?1,?Hu Hua et al..PMID: 38158022
, (2023),
PMID:
38158022
et al,Roxadustat ameliorates experimental colitis in mice by regulating macrophage polarization through increasing HIF level. In Biochim Biophys Acta Gen Subj on 2024 Mar by Guiping Kong, Hu Hua,et al..PMID:38158022
, (2024),
PMID:
38158022
Yes
