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Mouse Anti-Human CD8,PE Conjugated mAb

28286
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  Catalog No. Package Size Price
28286-1 25 Tests $249.00
28286-2 50 Tests $648.00
28286-3 100 Tests $888.00

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Description
  • Product Name Mouse Anti-Human CD8,PE Conjugated mAb
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 2A2
  • Isotype Mouse IgG2b, κ
  • Applications FC
  • Species Reactivity Hu
  • Specificity This antibody recognizes human CD8 in FACS.
  • Immunogen Description Human peripheral blood T cells
  • Formulation Lyophilized from a 0.2μm filtered solution in phosphate buffered saline (PBS) and reconstitute with sterile PBS .
  • Storage Store protected from light at 2-8˚C. Do not freeze. The expiration date is indicated on the vial label.
Application Details

Format:Antibodies are supplied in buffer containing stabilizer and 0.05% sodium azide
Preparation:This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human CD8 Recombinant Protein). The monoclonal antibody was purified from tissue culture supernatant or ascites by protein G affinity chromatography.
Product Notices:This reagent has been pre-diluted for use at the recommended volume per test. We typically use 1 106 cells in a 100-?l experimental sample (per test). An isotype control should be used at the same concentration as the antibody of interest.

Images
  • Mouse Anti-Human CD8,PE Conjugated mAb - SAB | Signalway Antibody

    Flow cytometric analysis of CD8 expression on Human peripheral blood mononuclear cells (PBMCs). PBMCs were stained with either mouse IgG2b, κ Isotype control or mouse anti-human CD8 antibodies conjugated to PE. Fluorescence histograms showing the expression of CD8 (or Ig Isotype control staining) were derived from events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a Beckman FC 500 Flow Cytometer System.

Product Description
CD8 (cluster of differentiation 8) is a transmembrane glycoprotein that serves as a co-receptor for the T cell receptor (TCR). Like the TCR, CD8 binds to a major histocompatibility complex (MHC) molecule, but is specific for the class I MHC protein. There are two isoforms of the protein, alpha and beta, each encoded by a different gene. In humans, both genes are located on chromosome 2 in position 2p12.The CD8 co-receptor is predominantly expressed on the surface of cytotoxic T cells, but can also be found on natural killer cells, cortical thymocytes, and dendritic cells. It is expressed in T cell lymphoblastic lymphoma and hypo-pigmented mycosis fungoides.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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